MS CM

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Questions related from MS CM

Will a sgRNA still work if it contains a reversed version of my sequences of interest (reversed as in 3' to 5' instead of the usual 5' to 3')?

Does directionality matter in this case? Will the said sgRNA still bind properly to my region of interest and recruit cas9? Thank you so much for your thoughts!

12 January 2017 4,838 1 View

Using confocal microscopy to detect fluorescence from cells in the cross-section of spheroids but could not pick up anything. What could be the issue?

The spheroids are made of MDA-MB-231 breast cancer cells which are themselves producing GFP-fusion proteins. Only very portions of the periphery can be seen by the confocal microscope, whereas the...

09 September 2016 606 3 View

What is the best preparation method for the sorting of MDA-MB-231 cells?

We have been sorting the breast cancer cell line MB-231. However, the yield has been pretty miserable. As you may have experienced, MB-231 cells either become clumpy or sink to the bottom during...

06 June 2015 9,305 8 View

What is the best preparation method for the sorting of MDA-MB-231 cells?

We have been sorting the breast cancer cell line MB-231. However, the yield has been pretty miserable. As you may have experienced, MB-231 cells either become clumpy or sink to the bottom during...

03 June 2015 5,221 7 View