Mrinal Das

2 Questions 4 Answers 0 Followers

Questions related from Mrinal Das

Hi, I want to overexpress a gene named SPRY4 in a transfectable cell line. Can anyone recommend me any easy and effective way to do that?

SPRY4 is a 4611 bp bp gene and the cell line I am going to use are testicular germ cell tumor cell lines NT2D1 and 833K. I have successfully done transient siRNA transfection with these cell...

11 November 2015 4,839 3 View

What stable system would be the best for overexpressing MyoD1. Lenti or piggybac?? in iPSC lines to make skeletal muscle

I have been considering to use either lentiviral or piggybac with Tet-On 3G promoter to overexpress MyoD1 in iPSC lines. I am pretty new in this stem cell research and I came to know from my...

01 January 1970 2,773 2 View