Mrinal Das

2 Questions 3 Answers 0 Followers

Questions related from Mrinal Das

What would be the best way to wet microfluidic device in order to get rid of air bubbles inside the chamber and channels?

Hello fellow researchers, I am trying to optimize the wetting procedures of microfluidic devices before using them as neuron and muscle culturing platforms. We have PDMS microfluidic devices with...

14 September 2020 2,044 6 View

Hi, I want to overexpress a gene named SPRY4 in a transfectable cell line. Can anyone recommend me any easy and effective way to do that?

SPRY4 is a 4611 bp bp gene and the cell line I am going to use are testicular germ cell tumor cell lines NT2D1 and 833K. I have successfully done transient siRNA transfection with these cell...

17 November 2015 2,701 2 View