Mrigya Babuta

2 Questions 9 Answers 0 Followers

Questions related from Mrigya Babuta

Can one use SP sepharose rather than Mono S for protein purification?

Hi, I am trying to purify a protein from its endogenous source. The protocol recommends the use of Mono S (a strong cation exchanger). Can I use SP sepharose fast flow (which is also a strong...

07 July 2015 6,299 9 View

How to Affinity Purify antibody from crude serum via western blot?

Hi, I am trying to affinity purify antibody from crude serum via western blot. I have used TBS-T for blocking and incubated with crude sera O/N at 4 on a rotative shaker. I eluted the antibody...

01 January 1970 4,802 10 View