Mozhgan Gholizadeh Vazvani

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Questions related from Mozhgan Gholizadeh Vazvani

What method is for the purification of DNA band stained with silver nitrate from acrylamide?

I want to extract and purify a band from an acrylamide gel stained with silver nitrate and send it for sequencing. Every method (including solution in water, solution in TE, crush and soak method)...

23 February 2024 1,028 2 View

Acetate magnesium can be replaced by other salts in "crush and soak" method?

I must extract DNA from polyacrylamide gels by "crush and soak" method and the DNA difusion solution contain 500 mM amonium acetate, 0.1% SDS, 10 mM magnesium acetate and 1 mM EDTA. Acetate...

15 January 2024 8,858 0 View