Mousumi Bhattacharyya

2 Questions 5 Answers 0 Followers

Questions related from Mousumi Bhattacharyya

How to achieve denaturation and renaturation of protein from pellet fraction of the cell (BL21 DE3)?

I am struggling with a Mycobacterium protein (phthalate esterase), which upon overexpression forms inclusion body (maybe) and it is found in the pellet fraction. I have tried cloning the protein...

28 July 2022 2,716 6 View

Lost protein (phthalate hydrolase) activity which I could achieve once but in later attempts the protein is not showing function? Please suggest??

I have cloned a phthalate hydrolase (~37 kDa) into a pMAL-c2x vector and overexpressed in E.coli BL21(DE3). Induction condition - 28 degrees for 3h with 0.5mM IPTG. This is a Mycolicibacterium...

08 June 2022 6,486 4 View