How to isolate chicken blood monocytes using histopaque gradient solution, and avoid the contamination by thrombocytes?

I tried isolation of chicken monocytes from blood using Histopaque 1.077 or 1.083 and centrifugation 800 xg for 15 min, washing by PBS 3 times at 200 xg. then culture in plastic dishes for 1 h...

24 May 2018 1,415 4 View