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Questions related from Moumita Sarkar
I need to inject progesterone and 17-beta estradiol to mice. Which solvent is the best choice for this ? Also please do mention the appropriate storage temperature for the preparation.
11 November 2018 5,747 0 View
I dump certain steroid treatments on my cancer cell lines followed by annexin staining. However even if i am observing under the microscope that there is significant cell death as all cells have...
06 June 2018 3,776 9 View
I am studying the expression pattern of few genes in tumor samples treated differently. Following cDNA making and PCR i can clearly see difference in gene expression on gel. However even though i...
09 September 2017 1,837 8 View
I have reconstituted progesterone in ethanol. I will further dilute it in sterile culture medium. Hence i was thinking of filtering the hormone preparation before i treat cells with it. Hence i...
08 August 2017 7,156 5 View
i have been trying to isolate DNA from mice blood from a long time using different kits. Presently i am using QIAGEN kit to isolate DNA . Initially i was getting nothing on the gel but after...
01 January 2017 7,153 2 View
