Motoharu Ono

2 Questions 5 Answers 0 Followers

Questions related from Motoharu Ono

Does anyone know a way to identify 2'O-ribose methylation sites or pseudouridylation sites of low abundant RNA using RT-PCR/qRT-PCR methods?

I’m trying to detect RNA modification of low abundant species in mammalian cells. I am wondering whether there are any protocols/kits for a high sensitivity and high throughput detection of RNA...

04 April 2013 944 3 View

Does anyone know a good protocol of negative staining of lenti-/adeno- virus for TEM?

I'm trying to take a TEM picture of my lenti-/adeno-virus using negative staining. However, we can only find a few viruses on a grid for some reason. I purified the virus and concentrated. I...

03 March 2013 3,252 7 View