Motahareh Nadimi

2 Questions 4 Answers 0 Followers

Questions related from Motahareh Nadimi

What is an extra band in gel electrophoresis of total RNA?

Hi, I am doing RNA extraction from human PBMC (of 1.5 ml blood) using Trizol Reagent. I got rations A260/280 1.8~1.95 and A260/230 1.7~2.2 and the concentration about 150~360 ng/ul. I have tried...

21 June 2019 3,311 6 View

What is the best way to homogenate PBMC with Trizol for RNA extraction?

I am doing RNA extraction using Trizol Reagent (RiboEx). I got rations A260/280 1.60~1.90 and A260/230 0.5~1.50 and also low yield about 70~160 ng/ul. I want to know if using liquid nitrogen and...

01 June 2019 7,226 5 View