Mostafa Jamshidiha

4 Questions 7 Answers 0 Followers

Questions related from Mostafa Jamshidiha

How to culture embryonic spinal cord neurons?

Hi I've been trying to isolate E15 spinal cord neurons. After isolation and digestion, I incubate the cells with Neural Base Media and 2% B27. Initially I used to add FBS to the media. Because...

09 September 2019 2,210 1 View

Which CCR7 and CD27 clones should I go for T-cell differentiation monitoring?

Hi I'm trying to study T-cell differentiation using a protocol from a paper. On the panel the authors have used anti-CD27–PE-Cy7 (clone 1A4CD27; Beckman Coulter) and anti-CCR7–FITC (clone...

11 November 2018 317 3 View

Whats the efficiency of T cell purification kits?

Hi I'm trying a purification kit from stemcell technologies (CD3 release kit). I used 100 million pbmc cells according to protocols. My initial flow cytometry results indicated I had around 38...

10 October 2018 1,985 4 View

MD simulations on stapled peptides?

Hi Im trying to build some stapled peptides for MD simulations on desmond. several papers have used schrodingers build module to build a helical peptide and then manually add the olefin staple but...

02 February 2016 9,806 1 View