4 Questions 10 Answers 0 Followers
Questions related from Moses Ekong
Does anyone have experience with Mouse Anti BrdU Immuno protocol? I am working on young rat's brains exposed to prenatal BrdU (i.p.). Literature available are confusing. Will I be required to...
04 April 2016 670 3 View
I tried CD11b antibody immunohistochemistry on frozen-section 4% PFA fixed tissues (post-fixed for 48hrs) using the following protocol: 1% H2O2 in PBS and washed. Preincubution in 5% Normal Horse...
09 March 2016 9,493 4 View
The tissue were perfusion-fixed with 4% PFA after PBS washing and the rat brains were remove and post-fixed for 48 hours. Will it be necessary or not to allow antigen retrieval before proceeding...
08 March 2016 7,844 3 View
Hello, I intend to prepare rat's fetal and pup brains for immunohistochemistry and immuofluorescence. I am required to inject the dams with BrDu, but I don't know how to prepare the solution. Can...
14 August 2015 8,194 2 View