Morgan Sedorovitz

3 Questions 2 Answers 0 Followers

Questions related from Morgan Sedorovitz

How can I optimize my transformation to get my pHelper plasmid to grow?

I have a pHelper plasmid that is 13kb. I have successfully gotten it to grow using DH5alpha cells in the past. There usually weren't many colonies and I would either need a large volume of culture...

10 July 2025 3,848 2 View

How to remove endotoxin from AAV purification pipeline?

We've been having an issue with endotoxin contamination in our AAV preps. A few months ago we had a very bad bacterial contamination which we have since addressed. Our preps are clean of bacteria,...

12 August 2024 9,244 1 View

Why am I getting a lot of very small colonies when I do a transformation?

I'm currently working on my fourth try of transformation. Every time the plates have A LOT of tiny colonies (photo attached). Every once in a while there is a larger colony, but when I screen...

07 March 2019 7,004 8 View