Why the White colonies in the blue/white screening of E.coli with pUC-18 vector appear bigger than the blue colonies?

I am performing a cloning experiment of a plasmodium gene with pUC-18 vector in E.coli (strain DH5alpha). After ligation when I am trying to analyze my vector+insert plate (X-gal+IPTG+LB-Amp), I...

30 October 2021 7,005 0 View