Monika Sharma

4 Questions 13 Answers 0 Followers

Questions related from Monika Sharma

Does anyone get full length ABCA1 protein band in Raw264.7 cell lysate?

I have been trying westren blot against anti-ABCA1 (tried Novus Biological and abcam antibodies both.). LXR activated cells do show 220kda ABCA1 band. But control cells show 120 KDa band exact...

10 April 2015 7,819 3 View

Does anyone have experience with HepG2 cells?

Something is happening in my lab. Cells were not split for a long time and then saw all flask are turbid and then some black tiny structures are overcrowded in all the flask. Next day, changed...

26 May 2014 177 3 View

What should the binding buffer composition be for binding purified protein with DNA?

I want to check whether or not my protein binds to DNA. At this stage, I am not using any labeling. I want to see if there is any DNA-protein interaction. Please suggest the ideal binding buffer...

03 April 2014 2,448 3 View

Any suggestions for treating DMSO soluble compound in cell culture?

I am using DMSO soluble compound to treat HepG2 cells. The problem is when I dilute DMSO soluble compound in water to get the desired concentration for treating cells, I am getting precipitation...

29 November 2013 6,198 21 View