Monica Martinez

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Questions related from Monica Martinez

Is it possible to elute RNA in 1mM EDTA instead of in DNase RNase free water for RNA clean up using RNeasy spin columns?

Does anyone elute RNA in 1mM EDTA? Downstream protocol has a 70ºC RNA denaturation step and the manual suggests to use EDTA to avoid RNA degradation? I am going to perform an RNA clean up using...

14 April 2015 2,751 3 View

Which kit for cDNA synthesis do you recommend to be used on bacterial RNA from soils? First strand cDNA from Roche, GoScript, High capacity cDNA?

I used First strand from Roche when working with yeast RNA, but it is too expensive compared with others. GoScript manual indicates that perform high yield RT also in the presence of inhibitors so...

23 October 2014 5,661 8 View