Monara Kaélle Angelim

3 Questions 10 Answers 0 Followers

Questions related from Monara Kaélle Angelim

Does someone have experience with ISH for neutrophins in embryo sections?

I've performed some ISH on embryo section for NT3, BDNF, NT4 and IGF, but the staining I've obtined is weak. I tried several changes in the protocol, (like using or not protK, differents...

04 January 2017 5,570 7 View

Is there any difference by using polyclonal or monoclonal secondary Ab for a monoclonal primary Ab?

Hi, does someone know if I do an IHF using IgG2b primary Ab, could I use polyclonal secondary Ab for it? or the result could be better if I use IgG2b secondary Ab, like the first mAb? Thanks in...

11 December 2015 3,819 11 View

Trouble in isolating monocytes from buffy coat

Hello everyone, I have read some questions in the forum about isolating monocytes from buffy coat, but I did not found explanation for my protocol not working lately. I have isolated monocytes...

01 January 1970 3,712 8 View