Momina Ejaz

3 Questions 2 Answers 0 Followers

Questions related from Momina Ejaz

Why do I get a smear when i preform the second round of a hemi nested PCR?

I have been dealing with degenerate primers for paramyxovirus virus detection. The detection assay calls for a heminested PCR with the same reverse primer used for round 1 and round 2 of the PCR...

24 June 2022 4,960 1 View

Why are multiple bands seen after PCR preformed with insert specific sequences on extracted plasmids?

We have 3 plasmids with 10+ inserts each, that we are trying to propagate. As a convention, DH5a was used for cloning purposes of the first plasmid. The resultant plasmid was verified with insert...

08 April 2022 8,790 3 View

Why non specific, multiple bands seen after plasmid miniprep ?

We have been trying to amplify our 2kb plasmid in the E.coli strain dh5a. The colony PCR after transformation (using primers targeting a 412bp region of the plasmid) yielded perfect sized bands....

25 January 2022 3,841 6 View