Molly Resnick

7 Questions 6 Answers 0 Followers

Questions related from Molly Resnick

Would SYBR Green dye interfere with pyro sequencing?

DNA was amplified using a PCR mix that included SYBR green dye. Could this DNA amplification undergo pyro sequencing without interference from the dye?

02 February 2016 2,669 5 View

What is causing these Taqman probes with the same sequence but different dyes to perform differently?

We are having a perplexing issue with some IDT double-quenched probes. We have a particular amplicon with a SNP and have control DNA that is wild-type, heterozygous, and homozygous for the SNP....

01 January 2016 7,081 3 View

How can I design probes in a GC poor region with a high enough Tm?

I am trying to use qPCR to genotype single nucleotide polymorphisms (SNP). Some of the SNPs are located in GC poor regions and I cannot get a probe Tm 8-10 degrees higher than my primer Tm. Do you...

06 June 2015 2,934 5 View

What are the alternate loci?

I am currently designing a SNP genotyping assay for CYP2D6 and I am confused about the alternate loci that keep appearing in my blast searches. What is an alternate locus? Does every gene have an...

03 March 2015 1,829 3 View

What does the allele CA|CA in the 1000 Genomes Browser mean?

Hi, I am trying to find homozygous mutant DNA for CYP2D6*6. SNPedia, ARUP, and the literature says this SNP is a T deletion at nucleotide number 1707 (according to M33388 sequence). When I go onto...

01 January 2015 1,469 1 View

If sanger results include nucleotides past my reverse primer does that imply I am amplifying unwanted regions?

I sequenced with both the forward and reverse primers in separate reactions (one tube forward primer + amplified DNA, and the other tube reverse primer + amplified DNA). The reverse reaction shows...

12 December 2014 2,912 1 View

Why does PCR with SYBR Green have a high fluorescence at cycle zero?

PCR was run with SYBR Green Taq and three different template DNAs that are wild type, heterogeneous, and homolozygous for a SNP. The only difference between the tubes is the template DNA. The...

12 December 2014 9,020 2 View