Mojtaba Zare Ebrahimabad

3 Questions 3 Answers 0 Followers

Questions related from Mojtaba Zare Ebrahimabad

How can the rehydration buffer of 2-D electrophoresis be avoided from crystallization?

I have prepared a stock of rehydration buffer containing 7 M urea, 2 M thiourea, 4% CHAPS, 1.5% SB-12, 65 mM DTT, 0.1% protease inhibitor cocktail, and 0.1% Ampholyte. Then, I aliquoted the...

08 January 2025 4,366 1 View

I did two-dimensional electrophoresis and got a lot of streaks and vertical lines instead of spots. Which problems make this figure?

I did IEF with 18 cm IPG and then a 25 cm SDS-PAGE for 6 hours. This picture shows my awful result, which has to be corrected. Does anybody have an idea? Note: The first line on the right side is...

03 January 2025 4,131 2 View

Why do I not have a spot in my SDS-PAGE after IEF?

I do a 2-dimensional electrophoresis for a human tissue. My steps were as follows: Whole protein extraction from 4 samples by grinding tissue in liquid nitrogen and solving proteins in rehydration...

24 December 2024 1,437 4 View