Does T-bet expression differ in Th1 and Th0 cells? What is the best protocol for polarizing Naïve CD4 T cells into Th1 cells?

I have isolated Naïve CD4 T cells from mouse spleen and tried to polarize them into Th1 cells. For doing that, I have coated a 48-well plate with 1 µg/ml Anti-CD3, 100 µl each well, incubated at...

18 February 2023 3,220 0 View