Mohsin Nawaz

5 Questions 5 Answers 0 Followers

Questions related from Mohsin Nawaz

Is it possible to demonstrate such type of results of western blot in publication? How to clear the background?

Hi I am doing western blot. After following the full length protocol, i examined the membrane on Biorad analyzer. The bands of my interested protein have poor visiblilty (14kDa). In addition,...

11 November 2019 1,575 7 View

Is there any way to differentiate host proteins (Histones) from the parasitic histones?

Hi i need little help. i have detected histones in the body of parasite. is there any way to differentiate between host proteins and parasitic proteins? as histones are nuclear proteins and...

10 October 2019 5,364 1 View

Did addition of 2X loading buffer to my protein samples will dilute the samples by 2?

Dear coordinates i need a little help. i have determined the concentration of protein and then added equal volume of loading buffer 2X. i want to make sure that loading buffer will dilute the...

10 October 2019 3,668 3 View

Hello. I need some help in case of PCR. my GOI is 661 bp. but may bands are coming close to 500, even less than 500 bp. what will be the problems?

i have added restriction sites ECO and XHO1. i have tried through all ways using different primers and softwares. what wll be the possible problems associated with it? I have also attached geldoc...

04 April 2019 4,364 13 View

Hi Dear i am now working on 5 Race 3 Race technology, i am just confused with designing of Gene specific primers. can anyone guide me how to design?

moreover, i have to use my own incomplete sequence for designing of gene specific primers. waiting for valuable suggestions Regards

03 March 2019 7,279 0 View