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Questions related from Mohsen Soltanshahi
I purified my protein by nickle column with FPLC. My base purification buffer had Tris 50 mM, NaCl 500 mM, Imidazole 40mM, urea 8M, 2Me 5mM at PH 8. I removed urea by gradient and eluted protein...
10 July 2019 153 8 View