2 Questions 13 Answers 0 Followers
Questions related from Mohsen Basiri
I recently tested PEI for production of GFP lentiviruses in HEK-293T cells. Important parameters in my protocol were as follows: - PEI solution (1 ug/uL): branched PEI (sigma408727) was diluted...
22 June 2014 8,425 2 View
I use lentiviral vectors (invitrogen pLenti6.3/TO/V5-DEST) for transgenesis of mESCs, but it is extremely inefficient in comparison with somatic cells. My mESC media is DMEM/F12+20%KOSR+LIF+R2i...
20 September 2013 2,192 3 View
