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I recently tested PEI for production of GFP lentiviruses in HEK-293T cells. Important parameters in my protocol were as follows: - PEI solution (1 ug/uL): branched PEI (sigma408727) was diluted...
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I use lentiviral vectors (invitrogen pLenti6.3/TO/V5-DEST) for transgenesis of mESCs, but it is extremely inefficient in comparison with somatic cells. My mESC media is DMEM/F12+20%KOSR+LIF+R2i...
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