Is there any method other than using the H++ sever to predict protonation states of proteins at a specific pH?

I've tried using the H++ server to generate protonated states of Acetylcholinesterase (pdb ID = 1GQR). But every time I try to load the generated file into AMBER (used pdb4amber) , I seem to get...

09 September 2017 8,415 5 View