Mohiraa Shafreen

5 Questions 9 Answers 0 Followers

Questions related from Mohiraa Shafreen

What should be the percentage of agarose for viewing cDNA?

I have isolated RNA from a few fish samples, and need to synthesize the first strand of cDNA and check if cDNA has been synthesized or not agarose gel electrophoresis has to be performed. How...

16 October 2023 760 6 View

How to overcome low yield of RNA from a plant sample?

While lysing the leaf sample for RNA isolation a jelly-like substance was released which led to a low yield of RNA. How to overcome this issue. PS: RNA isolation was carried out using nucleospin...

22 September 2023 8,373 3 View

Can anyone provide pro tips for isolating RNA from fish sample?

I need to isolate RNA from fish eggs and fish tissues. This is my first time dealing with fish samples. Can anyone help me out.

31 August 2023 9,323 4 View

What are the different bands after performing gel electrophoresis that can be seen for a PCR product?

Can anyone help me understand what do different bands mean in gel electrophoresis after performing PCR?

27 August 2023 4,316 4 View

What could have gone wrong while performing DNA isolation from insects?

I did DNA isolation for 4 different insect samples (species unkown), I got DNA for 1, whereas for the other three I couldn't isolate DNA, repeated the DNA isolation procedure 3 times using PCI...

26 August 2023 3,049 8 View