I am trying to do western blot of exosomes, however I am not getting any band even in whole protein staining. Can anyone help me?

I used 100ml of secretome to isolate the exosomes through ultracentrifugation. Then, I tried to lyse with 1X RIPA or 5%SDS along with 10s sonication. Later, added loading buffer of SDS-PAGE,...

14 August 2025 3,171 2 View