Mohd Faidz Mohamad Shahimin

2 Questions 5 Answers 0 Followers

Questions related from Mohd Faidz Mohamad Shahimin

Any advice on why in colony PCR using insert-specific primers and primers targeting vector DNA flanking the insert, I got different results?

I did cloning with pGEM-T kit and transformed into a DH5a cells. I've already grown the white colonies overnight and decided to check the insert before going for plasmid extraction and sequencing....

11 November 2015 7,757 3 View

Why did my positive clones not have insert?

Hi, I am quite new in cloning and have been trying very hard to troubleshoot cloning problems that I'm currently having. I am trying to create gene clone libraries for assA gene using pGEMt...

07 July 2015 8,103 11 View