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Hello everyone, I am working on a 73 kDa protein with a theoretical pI of 5.28 and encountering an issue after dialysis. I performed purification using 20 mM Tris buffer (pH 7.8) and eluted the...
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am performing a Threonine Synthase (TS) activity assay using a malachite green–ammonium molybdate-based phosphate detection method at 660 nm. The assay conditions include 100 mM TAPS buffer (pH...
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Good evening, My question is related to protein precipitation and analysis of its secondary structure; I have been facing this problem for the last 2 months. I have to do cd spectroscopy for the...
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