Mohd Javed

3 Questions 4 Answers 0 Followers

Questions related from Mohd Javed

Issue with Protein Concentration Increase and Tiny Pellet Formation After Dialysis (73 kDa Protein, pI 5.28) ?

Hello everyone, I am working on a 73 kDa protein with a theoretical pI of 5.28 and encountering an issue after dialysis. I performed purification using 20 mM Tris buffer (pH 7.8) and eluted the...

28 April 2025 1,810 7 View

Inconsistent Enzyme Saturation Curve at Fixed Substrate Concentration?

am performing a Threonine Synthase (TS) activity assay using a malachite green–ammonium molybdate-based phosphate detection method at 660 nm. The assay conditions include 100 mM TAPS buffer (pH...

15 March 2025 4,959 2 View

How to manage high ion concentrations during CD spectroscopy analysis of secondary structure of proteins?

Good evening, My question is related to protein precipitation and analysis of its secondary structure; I have been facing this problem for the last 2 months. I have to do cd spectroscopy for the...

09 November 2024 2,588 1 View