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Questions related from Mohd Faidz Mohamad Shahimin
I have amplified a functional gene with my environmental samples and already did the cloning and sequencing. I'm trying to cluster similar genes and I came across two term commonly used but I do...
23 January 2016 2,563 2 View
I did cloning with pGEM-T kit and transformed into a DH5a cells. I've already grown the white colonies overnight and decided to check the insert before going for plasmid extraction and sequencing....
22 November 2015 8,050 2 View
The protocol calls for 42 degree celcius. Would incubation at 45 degree celcius affect the transformation efficiency?
29 October 2015 5,597 6 View
I have run PCR for functional genes using AccuStart Tough mix II and have tried cloning before this and I did not get any successful cloning. The manufacturer master mix manufacturer claimed that...
17 September 2015 1,912 10 View
Hi, I am quite new in cloning and have been trying very hard to troubleshoot cloning problems that I'm currently having. I am trying to create gene clone libraries for assA gene using pGEMt...
10 July 2015 6,727 10 View