Mohd Faidz Mohamad Shahimin

6 Questions 12 Answers 0 Followers

Questions related from Mohd Faidz Mohamad Shahimin

What's the difference between OTU and contig when clustering DNA sequences?

I have amplified a functional gene with my environmental samples and already did the cloning and sequencing. I'm trying to cluster similar genes and I came across two term commonly used but I do...

23 January 2016 2,621 2 View

Any advice on why in colony PCR using insert-specific primers and primers targeting vector DNA flanking the insert, I got different results?

I did cloning with pGEM-T kit and transformed into a DH5a cells. I've already grown the white colonies overnight and decided to check the insert before going for plasmid extraction and sequencing....

22 November 2015 8,099 2 View

Can ligation (pGEM-T) at 16 C overnight increase ligation efficiency?

I've tried incubation at 4 C overnight as recommended in the protocol but I heard incubation overnight at 16 C can increase the success rate of ligation?

05 November 2015 2,720 5 View

Would transformation of competent cells (DH5α) at 45 degree celcius affect the transformation efficiency?

The protocol calls for 42 degree celcius. Would incubation at 45 degree celcius affect the transformation efficiency?

29 October 2015 5,637 6 View

Does overnight storage at 4 degree celcius degrade the A-tailing from amplicons?

I have run PCR for functional genes using AccuStart Tough mix II and have tried cloning before this and I did not get any successful cloning. The manufacturer master mix manufacturer claimed that...

17 September 2015 1,949 10 View

Why did my positive clones not have insert?

Hi, I am quite new in cloning and have been trying very hard to troubleshoot cloning problems that I'm currently having. I am trying to create gene clone libraries for assA gene using pGEMt...

10 July 2015 6,781 10 View