Mohd ZIAUDDIN Ansari

5 Questions 9 Answers 0 Followers

Questions related from Mohd ZIAUDDIN Ansari

Suitable reducing agent for Circular Dichroism (TCEP, DTT, B-ME)?

I work on a protein that readily forms dimer. I want to study the monomeric form through Circular Dichroism (CD). Reducing agents are known to interfere with CD.Please suggest what will be the...

01 November 2016 7,635 2 View

Can I lyophilise protein after in vitro phosphorylation by casein kinase 2?

I want to do in vitro phosphorylation of 8kDa protein by Casein kinase 2 and after phosphorylation wants to its lyophylization . Does it affect its structure ? or can I store it at -80 degree...

06 August 2015 3,717 4 View

What will be easy technique to detect phosphorylation and pyrophosphorylation in protein?

I am looking for detection of phosphorylation and pyrophosphorylation on protein. Please suggest me simpler technique other than Mass spectrometry, western blotting and autoradiography.

28 December 2014 4,025 5 View

Can anyone help me with the problem in Protein expression and lysis buffer?

I am expressing 6xHis tagged 8.5KDa human Protein (pI~4.19) which cloned into pET21b expression vector and express in BL21 DE3 Codon plus strain. I see expression when I mix bacterial pellet with...

24 December 2014 336 12 View

Why do we mostly use only 6xHis Tag to purify Proteins Not 8xHis Or 10xHis?

When ever people make a clone with His Tag to their protein for purification by affinity chromatography why they mostly use only 6 His to protein. What will happened if we put more His suppose 8...

16 September 2014 8,425 12 View