Mohammed Moudjahed

3 Questions 4 Answers 0 Followers

Questions related from Mohammed Moudjahed

When carboxylic acids are injected in an HPLC C18 column and a UV detector is used, what will be detected? The acid or its conjugate base?

As we know, carboxylic acids are generally weak acids so they are not completely dissolved in water (aqueous solution), as a result, when we inject the carboxylic acid there must be some amount of...

03 March 2015 6,514 7 View

What is the best way to adjust the pH of an ammonium acetate buffer solution for an injection in an HPLC C18 column?

1) When preparing an ammonium acetate 5mM buffer solution with pH=3.3, which is better to use to adjust the pH? An inorganic acid (HCl) or the corresponding organic acid (CH3COOH)?2) Is it...

02 February 2015 8,924 12 View

Can an HPLC C18 column stand an injection of aliphatic carboxylic diacids such as glutaric and succinic acids?

The pH of the solution obtained (containing the diacids) is 2.33, and the method says we need to prepare a buffer solution of ammonium acetate 5mM with a pH=3.3 as 70% of the mobile phase, the...

02 February 2015 6,647 6 View