Mohammed Toufiq

4 Questions 3 Answers 0 Followers

Questions related from Mohammed Toufiq

How to normalize and handle batch effect of qRT-PCR data?

Hi, I have a question on qRT-PCR gene expression data analysis. I have a gene panel with 300 genes (292 test genes) + 8 housekeeping genes. The samples were run in batches over the period of time...

03 June 2020 6,149 1 View

Support for import of custom matrix data in the ddCt R package?

Hi, I would like to know if I can import the data into the ddCt package in the matrix format for the analysing the qRTPCR data. If possible, how could this be done? I have a .txt file with 260...

20 August 2019 1,631 2 View

qRT-PCR data analysis queries?

Hi, I have a question on qRT-PCR data analysis. The gene panel has more than 200 genes along with the multiple reference/housekeeping genes. I have 50 paired samples (i.e Before vs After Vitamin...

13 June 2019 7,320 3 View

RT-qPCR: How to perform calculation of pairwise variations to determine the optimal number of reference genes?

Hi, I am using geNorm, Normfinder, RefFinder, and BestKeeper to identify and select the appropriate reference genes for the normalization in the RT-qPCR analysis. I was trying to identify the...

09 April 2019 6,766 3 View