Mohammed Abdelbaset

3 Questions 1 Answers 0 Followers

Questions related from Mohammed Abdelbaset

If I removed the Plasmid ATG to prevent tag expression, the expression would start at ATG of my desired protein?

I am using pET-51b vector and I do not want the tags to be expressed with my gene. so I want to design primer to remove Plasmid ATG: 1- ATG (plasmid)-tag_ ATG (desired gene) 2- (plasmid without...

09 December 2019 7,374 4 View

Can the insert gene leave the plasmid after cloning?

after doing succefel ligation and transformation to Ecoli BL21, I made glycerol stock. Then when I retured, I found the plasmid without the insert How is this possible?

02 October 2019 4,784 3 View

How to carry out assay for activity of non-purified Carbonic Anhydrase ?

I expressed CA in E-coli and I want to make sure the expressed protein is carbonic anhydrase, is there any test to determine the activity of non purified CA ? Thanks in advance

25 March 2019 4,471 1 View