What is the reason for the variation in reading of standards set from a stock of a plasmid by the Real-Time PCR?

Dear All, To measure the DNA copy number of HBV virus, I prepared a standard series from the HBV-containing plasmids stock (10 +5, 10 +4, 10 +3, 10 +2, 10 +1), and after reading with Real-Time...

08 August 2019 2,953 4 View