Mohammad Nasiruddin

3 Questions 6 Answers 0 Followers

Questions related from Mohammad Nasiruddin

Why i am not getting Log filename to run AutoGrid ???

I am new to molecular docking and trying to dock using ADT, MGL tools, Autodock Vina. I have prepared ligand and protein to run autogrid. But in the parameter file , it is taking the gfg file. but...

09 September 2017 2,833 2 View

I am not getting band of proteins in nitrocellulose membrane , but standard protein (Western Blot). Could you suggest me things to change or not ??

As i am facing this problem, i changed the homogenate (kidney) and made new. For electroscopes , i used 10% SDS-PAGE and sample volume was 50 micogram/10 micro lit.. For transferring protein i set...

06 June 2016 9,767 7 View

How can i do pyogallol based SOD1 for Kidney tissue homogenate except assay kit ??

I have tried to do using 1ml Tris-EDTA Buffer , pH -8.2, tissue homogenate 50 micro lit and 1 ml pyrogallol (0.2 mM). But results is not good as rate of change for homogenate is higher for some...

02 February 2016 4,640 2 View