Mohammad Ishaq

5 Questions 16 Answers 0 Followers

Questions related from Mohammad Ishaq

What ratiometric method can be used to detect cytosolic calcium release after 12 hours of stimulation?

Hello guys, I am doing some experiments which involve increase of cytosolic calcium. At present i do not know what is the source of this increased calcium in cytosol. So i just wanted to confirm...

07 July 2015 2,886 4 View

Optimum Number of cycles in a PCR ?

The number of cycles required for amplification depends on the number of copies of template DNA present at the beginning of the reaction. But what is the ideal number of cycles. Also if more no of...

02 February 2015 5,415 5 View

Do cancerous cells and normal cells have same CHROMOSOMAL TERRITORY pattern?

Chromosomes are now well known to be located in their specific positions within the nucleus. Different CHROMOSOMAL TERRITORIES hold some specific chromosomes. So if some genes are within a...

12 December 2014 7,222 5 View

Can anyone suggest good protocols for ROS measurement by DHE and DCFDA by FACS?

I am currently measuring ROS in T24 bladder cancer cell after drug treatment. I have already measured ROS generation by microscopy using cmDCFDA. It is confirmed that my drug induces ROS as NAC...

10 October 2013 607 3 View

Dot-like particles in cell culture: is it contamination or not? No change in pH, turbidity, cell morphology and cell growth.

I am experiencing some strange problem in some adherent cancerous cell lines. I have observed that these dots only adhered to cells for first 2 days after passaging, but these dots then start to...

01 January 2013 2,821 88 View