Mohammad Taghizadeh

4 Questions 2 Answers 0 Followers

Questions related from Mohammad Taghizadeh

Some problem in CT in the qPCR?

Hi we have done qPCR we some specific gene and we got best melting curve and amplification curve but there is some problem is CT and we can not access to CT and in the heatmap all of the positions...

27 December 2023 553 0 View

Analyzing of qPCR melting curve?

Hi I am doing qPCR over and over again exactly based on its protocols and I sure about quality of primers but every time that I am doing qPCR I cant not get sharp pick some times I have two picks...

21 November 2023 6,886 2 View

How can I design Primers for piRNAs?

I want to design Primers for piRNA-823 but all of related articles just mentioned the forward primer but I want to know the method of designing primers is it designed by stem-loop or poly A and...

20 November 2023 1,552 3 View

How can I fix Multiple pick in melting curve?

I did qPCR and I get two or pick in melting curve specially less than 78 degree and I redid multiple time but got exactly similar results I do not know what is the problem

01 November 2023 5,380 0 View