Mohammad Taghizadeh

3 Questions 2 Answers 0 Followers

Questions related from Mohammad Taghizadeh

Analyzing of qPCR melting curve?

Hi I am doing qPCR over and over again exactly based on its protocols and I sure about quality of primers but every time that I am doing qPCR I cant not get sharp pick some times I have two picks...

21 November 2023 6,853 2 View

How can I design Primers for piRNAs?

I want to design Primers for piRNA-823 but all of related articles just mentioned the forward primer but I want to know the method of designing primers is it designed by stem-loop or poly A and...

20 November 2023 1,531 3 View

How can I fix Multiple pick in melting curve?

I did qPCR and I get two or pick in melting curve specially less than 78 degree and I redid multiple time but got exactly similar results I do not know what is the problem

01 November 2023 5,351 0 View