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Questions related from Mohammad Kawsar Manik
One of the molecules of my crystal structure has density for the two conformations of a loop. I want to build the alternate conformation of the loop using coot. However, haven't figured it out how...
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I am working on Ankyrin repeat domain of a protein and it produces a good shaped crystals. However, at the beginning it was twinned. Thus, I tried to crystallized in low temperature with some...
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I tried to purify a fungal protein using E. coli BL21 DE3 strain. Unfortunately, it has very low expression.
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Currently, I am purifying a protein which has a fairly good concentration after elution. However, after size exclusion chromatogrphy, protein is aggregated. What can be the possible solution?
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