Mohammad Kawsar Manik

4 Questions 15 Answers 0 Followers

Questions related from Mohammad Kawsar Manik

How to build alternate conformation of loop in coot?

One of the molecules of my crystal structure has density for the two conformations of a loop. I want to build the alternate conformation of the loop using coot. However, haven't figured it out how...

10 January 2019 1,453 2 View

Is there any way to improve the diffraction of a protein crystal?

I am working on Ankyrin repeat domain of a protein and it produces a good shaped crystals. However, at the beginning it was twinned. Thus, I tried to crystallized in low temperature with some...

18 May 2015 7,640 9 View

How can I increase the expression level of a protein?

I tried to purify a fungal protein using E. coli BL21 DE3 strain. Unfortunately, it has very low expression.

08 December 2014 361 8 View

How can I prevent the oxidation of a protein after elution?

Currently, I am purifying a protein which has a fairly good concentration after elution. However, after size exclusion chromatogrphy, protein is aggregated. What can be the possible solution?

03 November 2014 9,670 10 View