Mohammad Kawsar Manik

5 Questions 16 Answers 0 Followers

Questions related from Mohammad Kawsar Manik

How to build alternate conformation of loop in coot?

One of the molecules of my crystal structure has density for the two conformations of a loop. I want to build the alternate conformation of the loop using coot. However, haven't figured it out how...

10 January 2019 1,460 2 View

Is there any way to improve the diffraction of a protein crystal?

I am working on Ankyrin repeat domain of a protein and it produces a good shaped crystals. However, at the beginning it was twinned. Thus, I tried to crystallized in low temperature with some...

18 May 2015 7,643 9 View

How can I increase the expression level of a protein?

I tried to purify a fungal protein using E. coli BL21 DE3 strain. Unfortunately, it has very low expression.

08 December 2014 363 8 View

How can I prevent the oxidation of a protein after elution?

Currently, I am purifying a protein which has a fairly good concentration after elution. However, after size exclusion chromatogrphy, protein is aggregated. What can be the possible solution?

03 November 2014 9,673 10 View

How can I recover protein from inclusion bodies?

I want to purify a protein in a soluble form. However, due to manifolding all of my desired protein is in the insoluble portion. Is there any method to recover this protein in soluble form?

09 October 2014 1,569 9 View