Mohammad Azhar Aziz

3 Questions 7 Answers 0 Followers

Questions related from Mohammad Azhar Aziz

Do we need to keep replenishing the selection antibiotic in the media while selecting for colonies?

After transfection with CRISPR/shRNa/ORF construct and selection for colonies, it usually takes 3-6 weeks or more to obtain single colonies. Do we need to keep replenishing the antibiotics and...

23 July 2020 4,691 2 View

Why can't an anonymous peer review model be followed?

The manuscript that comes for review has its authors and affiliations mentioned which is a source of bias that can impact the review process. Why don't we just send the manuscript without...

20 November 2013 3,429 8 View

What is the best way to store OCT embedded fresh tissue samples from the gastrointestinal tract of humans?

I am collecting samples and embedding them in OCT for future cryosctioning and other studies. What is the best way to store and what is the maximum duration?

09 December 2012 7,822 1 View