Mohammad Alkhrayef

2 Questions 4 Answers 0 Followers

Questions related from Mohammad Alkhrayef

Monocytes differentiation to M1 and M2 phenotypes Challenge. what went wrong?

I tried to differentiate monocytes toward M1 and M2 phenotypes. The method I followed: Firstly, I isolated monocytes from blood cones using Pan Mono isolation kit, human (MACS), Miltenyi. Then, I...

09 September 2020 4,230 2 View

Polarize monocytes to M1 or M2 protocol?

I'm trying to polarize human monocytes to M1 or M2 phenotypes. I cultured them for 5 days in RPMI medium +10% FCS with 50 ng/ml GM-CSF (for M1) and RPMI medium +10% FCS with 50 ng/ml M-CSF (for...

03 February 2020 9,887 6 View