Mohamed Abd Elhamid Tantawy

2 Questions 6 Answers 0 Followers

Questions related from Mohamed Abd Elhamid Tantawy

Is there any problem with keeping the PBMCs in a culture medium for 24 hr at 37c before doing TLRs stimulation experiment?

Hello all, I am isolating fresh PBMCs from healthy donors to do some gene expression studies after infection with viruses or stimulation with TLRs agonist. My question is, can I keep the...

07 July 2014 2,616 6 View

Does pCMV6-entry induce cytokines that could reduce influenza virus replication in A549 cells?

I would like to ask about the transfection of A549 cells. I used pCMV6-entry plasmid to overexpress one gene of interest (I want to study its role in inhibiting influenza virus replication) in...

05 May 2014 9,551 3 View