Mohamed Mahgoub

2 Questions 13 Answers 0 Followers

Questions related from Mohamed Mahgoub

Can anyone suggest a stable cell line with Neon electroporation?

I am trying to stably transfect B cell line using Neon device, but during optimization of my experiments I noticed that most cells die after 72 hours (although I got about 60% positive cells with...

04 April 2014 1,113 5 View

What is the best polymerase with the least need for optomization for amplification of tiny template in precious sample?

I want to sequence pro-viral DNA from precious small sample (about 9 kb), The copy number of this pro-virus is very tiny. I am going to amplify several PCR products that cover all pro-virus and...

02 February 2014 794 35 View