Mohamed Elfeky

3 Questions 7 Answers 0 Followers

Questions related from Mohamed Elfeky

What is the right time of protein expression using tetracycline-regulated Tet-off system?

I am using a tetracycline-regulated Tet-off system (Doxycycline) to express my target protein in MKN28 and WT-A10 cells. I asking which is better to induce expression at culturing (0-time point)...

02 February 2017 5,259 2 View

Does anyone know what is the best way to prepare HMGB1 A and B Box domain separately?

I had prepared recombinant HMGB1 full length protein and I'm planning to use A and B box . Is there any possibility of proteolytic cleavage of full protein or I have to clone and express each...

09 September 2013 3,059 7 View

Purification of adiponectin from bovine serum?

I'm trying to purify adiponectin from bovine serum following the same method of using zinc chelating sepharose column. I ordered IMAC sepharose TM Fast flow column (GE healthcare 28-4046-25 AD)...

09 September 2013 7,275 2 View