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During protein concentration step by using Amicon tubes with 10KDa cutoff (from Merck company), I could see some cloudy suspension which indicate for protein aggregates formation. Afterwards in...
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As I mentioned my bands are sharp and perfect but no free oligonucleotides and I repeated it again using a large volume of labelled probe but I got the same result no free oligonucleotides. Why?
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My collegues using the same protocol and same buffers are getting good results.
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