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I've read my MTT assay on 450 nm and 620 nm as reference. Can you help me how to calculate the CCI 50 for both the virus and the extract? The reading on 620 was much higher than on 450. Would you...
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The Elisa reader I'm using doesn't have the desired wavelength 570 nm or the range 500-600 , but it has other lengths 490,630,390,450,405 , which of them i can use for the assay on MDCK cells?
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