Mohamed Ibrahim

2 Questions 2 Answers 0 Followers

Questions related from Mohamed Ibrahim

Do the freezing reduces the efficacy of the cells for the electroporation ? should i decrease the voltage for the next time?

I'm making reprogramming of MEFs experiment and I used Piggy bac, Sleeping beauty and episomal transposition systems. After electroporation i froze the cells, then i thawed the cells but there...

07 January 2021 5,659 2 View

Reprogramming of IPs cells problems ?

I'm making reprogramming of MEFs experiment and I subcultured the cells twice then I used Piggy bac, Sleeping beauty and episomal transposition systems to compare between the efficiency of each...

01 January 1970 4,937 1 View