Mohamed Hasanin

5 Questions 5 Answers 0 Followers

Questions related from Mohamed Hasanin

Can someone help me with my thesis please?

Dear connections, I am undertaking a master program in pharmacology and as so far progressed with my thesis writing and nearing completion. I am kindly seeking if anyone is interested in helping...

25 March 2025 7,378 5 View

Based on 400 and 320 and 280 absorption can i suspect aggregation?

before the O/N labeling with cy dyes, 280 absorption was lets say 10 times 320 after removing the free dye the 280 to 320 ratio became 5 but 320 did not increase just that the sample got diluted...

12 January 2025 8,538 1 View

I really wish to know when would small molecules specifically DTT start to elute from PD columns saying my sample was 0.5 ml or whatever?

like i know for proteins bigger than 5 kd will start to elute immediately after the void volume which is the volume occupying the spaces between the beads right? so if i am putting 1 ml the...

07 January 2025 1,212 0 View

Confused about PD-10 column?

Can someone please explain what is the difference between the bead volume and the column volume? If they are the same it says on the pd-10 column booklet that the bed volume is 8.3 so assuming...

07 January 2025 2,044 0 View

Is there any possible way I can tell whether the presence of more bands in the elutions are because insufficient washing or truncated/degraded protei?

Saying I only have the protein purification gel report for 3 different preps with total soluble flow through and fractions 1-5 eluted with imidazole so is there any possible way I can tell whether...

31 December 2024 3,724 8 View