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Questions related from Mohamad Farhan Bin Mohamad Sobri
For inoculum prep, do we (a) measure growth at OD600nm and stop when said OD value reaches 0.5. Inoculation volume as set beforehand. or (b) do we take a certain amount of culture, and...
01 September 2016 1,924 3 View
Hello,I am writing to ask on how to maintain positive transformants of yeast strains that are originally auxotrophic in a rich medium such as biomass hydrolysate. To my understanding, selection of...
23 January 2016 7,438 0 View
Hello there,I am writing to ask for insight as to how best to express my heterologous gene of interest extracellularly from Saccharomyces cerevisiae. The recombinant strain is then required to...
08 December 2015 6,443 2 View
Dear all,I would like to know if a regular off-the-shelf baking yeast can be used for laboratory scale heterologous protein expression while at the same time retaining it's ability to ferment...
05 November 2015 4,127 4 View
Dear all, I am having trouble understanding the following statement as I understand both to be interchangable. I would appreciate it if someone could clarify as to what they actually mean."The...
13 October 2015 4,953 3 View
Dear all, I would like to ask for general recommendations to anyone with experience of using the aforementioned kit for their RACE PCR.I am currently having difficulties on using the kit for both...
14 September 2015 3,769 8 View