Mohamad Farhan Bin Mohamad Sobri

3 Questions 2 Answers 0 Followers

Questions related from Mohamad Farhan Bin Mohamad Sobri

Prepare the appropriate amount of culture for a final OD600= 0.5. what does this mean?

For inoculum prep, do we (a) measure growth at OD600nm and stop when said OD value reaches 0.5. Inoculation volume as set beforehand. or (b) do we take a certain amount of culture, and...

01 September 2016 1,888 3 View

Vector for expression and secretion of heterologous peptide in Saccharomyces cerevisiae?

Hello there,I am writing to ask for insight as to how best to express my heterologous gene of interest extracellularly from Saccharomyces cerevisiae. The recombinant strain is then required to...

08 December 2015 6,413 2 View

Anyone familiar with SMARTER RACE 5' and 3' Kit?

Dear all, I would like to ask for general recommendations to anyone with experience of using the aforementioned kit for their RACE PCR.I am currently having difficulties on using the kit for both...

14 September 2015 3,735 8 View