Moe Mahmoud

9 Questions 1 Answers 0 Followers

Questions related from Moe Mahmoud

What is a reliable positive control for Immunofluorescence on deparaffinized mouse tissues?

I am doing IF on deparaffinized mouse tissues, (original tissues, are vertical cut mouse tissue sections ; mouse was cut vertically several times, side by side, and tissues were paraffinized)....

02 February 2015 7,734 2 View

Why do the stacking gel wells rupture upon sample loading during PAGE ?

Hi guys, Hope everything is going well with you. So I am encountering an issue, in which select (usually one or two) stacking gel wells rupture following loading the samples (usually containing 10...

12 December 2014 7,121 9 View

How do I over-express a protein ?

Hi Guys, hope all is well with you. I want to overexpress a protein using HEK 293 Cell line. Could you please kindly tell me what is the most convenient way to do so. I read something about...

11 November 2014 4,181 2 View

What happens if the beads volume used in Immunoprecipitation was lower than suggested ?

If the beads volume used was lower than suggested (normal), would the immunoprecipitation not work at all, or it will work but the amount of protein precipitated will decrease, and hence fainter...

10 October 2014 6,979 2 View

How many times can I thaw 1 ml DTT aliquot, forzen at -20 degress ?

Hi guys, so in order to prepare stripping buffer to strip my Western blot membranes, I need to use DTT. We have in the lab DTT stored as 1 ml aliquots, and frozen at -20. I already thawed one...

10 October 2014 4,651 2 View

How to handle a frozen PVDF membrane, which hasn't been treated with any antibodies yet ?

Hi guys, so after blotting the membrane, and checking for total protein content, I washed the membrane for 20 mins in blocking buffer to remove ponceau s stain, then washed in distilled water for...

10 October 2014 6,317 2 View

Can anyone help with the calculation of the protein sample volume before electrophoresis?

Hi, hope all is well with you guys. I have a question regarding the calculation of the volume of the protein sample prior to the loading for electrophoresis. I always make sure to load the same...

09 September 2014 6,844 5 View

Can anyone help me resolve a 10% separating gel, for PAGE, surface issue ?

Hi guys, So while carrying out PAGE, following the addition of propanol alcohol, the surface becomes smooth, but not straight, it usually starts to take a lower angle near the end. It's not a huge...

08 August 2014 1,824 13 View

Can anyone help with a question about Post Bradford Assays and Protein PAGE?

Hi guys, hope all is well, so I just finished bradford assay on three different cell lysates. The chosen protein concentrations after analyzing the results were 3.5362 ug/ul, 0.6579 ug/ul, and...

08 August 2014 6,606 11 View